Figure 5.
Histopathological results of the skin of normal and photoaged groups using Masson trichrome staining and immunohistochemical labeling. (A,B) Histopathological results of Masson staining of skin from normal and photoaged groups. The collagen volume fraction was calculated, which is expressed as mean ± SD of at least three measurements (*p < 0.05, **p < 0.01, ***p < 0.005). (C,D) Immunohistochemical markers for Collagen I, HSP27, p62 and LC3 B and quantification of Average Optical Density (AOD) were performed on normal and photoaged skin, and the results are expressed as mean ± SD of at least three measurements (*p < 0.05, **p < 0.01, ***p < 0.005).