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. 2021 Feb 5;9:633331. doi: 10.3389/fcell.2021.633331

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Copyright © 2021 Bai, Wang, Huang, Wang, Cao, Liu, Liu, Li and Chen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Histopathological results of the skin of normal and photoaged groups using Masson trichrome staining and immunohistochemical labeling. (A,B) Histopathological results of Masson staining of skin from normal and photoaged groups. The collagen volume fraction was calculated, which is expressed as mean ± SD of at least three measurements (*p < 0.05, **p < 0.01, ***p < 0.005). (C,D) Immunohistochemical markers for Collagen I, HSP27, p62 and LC3 B and quantification of Average Optical Density (AOD) were performed on normal and photoaged skin, and the results are expressed as mean ± SD of at least three measurements (*p < 0.05, **p < 0.01, ***p < 0.005).