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. 2021 Feb 18;4:229. doi: 10.1038/s42003-021-01745-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a–c Expression of mouse endogenous CLEC18A (mCLEC18) mRNA and protein in B6 mice was determined by RT-PCR (a) and WB (b, c). 1: Brain, 2: Lung, 3: Heart, 4: Thymus; 5: Liver, 6: Spleen, 7: Kidney, 8: Bone marrow; 9: 293T cells transfected with pCMV-Tag4A (vector control); 10: 293T cells transfected with pCMV-Tag4A-mCLEC18 (50 kDa). Arrow indicates the position of mouse CLEC18 (n = 3). d Expression of human CLEC18A and CLEC18A (S339R) in ROSA-CLEC18A and CLEC18A(S339R) mice was determined by PCR. 1: Brain; 2: Lung; 3: Heart; 4: Liver; 5: Spleen; 6: Kidney; 7: Bone marrow; 8: Splenocyte (n = 3). e Detection of human CLEC18 protein (50 kDa) in mouse bone marrow-derived dendritic cells (BMDCs), as well as macrophages (BMDMs) cultured under M-CSF and GM-CSF, respectively. 1: Wild type mice (WT); 2: ROSA26-CLEC18A mice; 3. ROSA-CLEC18A(S339R) mice. Arrow indicates the molecular weight (50 kDa) of human CLEC18A/CLEC18A(S339R) (hCLEC18) (n = 3).