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. 2021 Feb 18;4:229. doi: 10.1038/s42003-021-01745-7

Fig. 2. CLEC18 enhances IFN production and ISG expression to suppress virus replication.

Fig. 2

a BMDMs (1 × 106/well) from WT, ROSA-CLEC18A, and ROSA-CLEC18A(S339R) mice were incubated with HMW poly (I:C) for 24 h, followed by harvesting cells to determine the expression of cytokines by ELISA (n = 3–6). b, c BMDMs (1 × 106/well) from WT, ROSA-CLEC18A, and ROSA-CLEC18A(S339R) mice were incubated with RNA viruses (H5N1 MOI = 2; DV MOI = 30) for 24 h, followed by harvesting cells to determine cytokines expression by ELISA (n = 3–10) (b) and IFN-stimulating genes (ISG) expression by quantitative RT-PCR (n = 3) (c). d Virus replication was determined by plaque assay (left), and quantitative RT-PCR to determine the expression of virus structure genes (M) and nucleoprotein (NP) (right) (n = 3–10). One-way ANOVA was performed. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001, for WT group versus group of CLEC18A or CLEC18A(S339R). H5N1 type A influenza virus, DV dengue virus.