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. 2021 Feb 18;4:229. doi: 10.1038/s42003-021-01745-7

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Scheme of human TLR3 deletion mutants. b–g The 293T cells (3 × 10⁶) were transfected with pMACS-Kk-HA-hCLEC18A/CLEC18A(S339R) (7.5 μg) and pFlag-CMV1-hTLR3 (full-length and deletion mutants) (7.5 μg) for 48 h, followed by immunoprecipitation using anti-Flag mAb. Immunoprecipitates were fractionated on SDS-PAGE and blotted to PVDF membranes before probing with anti-HA mAb and anti-Flag mAb, respectively. Blots were further incubated with peroxidase-conjugated anti-mouse IgG antibody and developed by ECL Kit. h–k The 293T cells (3 × 10⁶) were transfected with pCMV-Tag4A-hCLEC18A (full-length and deletion mutants) and pUNO1-TLR3-HA for 48 h, followed by immunoprecipitation using anti-Flag mAb. Immunoprecipitates were fractionated on SDS-PAGE before blotting to PVDF membranes. Blots were probed with anti-HA mAb and anti-Flag mAb, respectively, followed by incubation with peroxidase-conjugate anti-mouse IgG antibody and developed by ECL Kit. Iso isotype control antibody, IP immunoprecipitation, WB western blot. Red arrows: CLEC18 or TLR3; V: vector only; [+]: Flag-tagged CLEC18A and deletion mutants were co-transfected with HA-tagged TLR3 expression vector (TLR3-HA). All WB experiments have been repeated at least three times.