Fig. 8. CLEC18A and CLEC18A(S339R) protect host from IAV-induced inflammation and lethality.
a, b WT, ROSA-CLEC18A, and ROSA-CLEC18A(S339R) mice were inoculated with H5N1 virus (1.5 × 103 PFU) by intranasal route to observe body weight changes (a) and survival rate (b) (n = 21–28). c Lung tissues harvested at day 4 post infection were subjected to hematoxylin and eosin staining (n = 3). d, e Virus titer in lung tissues was determined by plaque assay (n = 4–6) (d), while viral structure gene expression (M and NP) was determined by RT-PCR (n = 4–6) (e). f Cytokines contained in the bronchial alveolar lavage fluid (BALF) from WT and ROSA-CLEC18A/CLEC18A(S339R) mice at days 4 and 7 post infection were determined by ELISA (n = 3–17). d.p.i. days post infection. One-way ANOVA was performed. *P < 0.05, **P < 0.01, ***P < 0.001 and ****P < 0.0001 for WT group versus group of CLEC18A or CLEC18A(S339R). Scale bar: 200 μM.