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. 2021 Feb 18;12:1142. doi: 10.1038/s41467-021-21476-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Western blot of ChAT in BF, CPu, PM, and PFC. b Acetycholine content in BF and CPu examined by HPLC-MS/MS. c Extracellular acetycholine level in the medial prefrontal cortex collected by microdialysis and examined by HPLC-MS/MS. d Threshold current to elicit action potential with the increase of injected currents in NBM cholinergic neurons recorded by whole-cell patch-clamp. Spike numbers with the increase of injected currents in NBM cholinergic neurons recorded by whole-cell patch-clamp. e Threshold current to elicit action potential with the increase of injected currents in HDB cholinergic neurons recorded by whole-cell patch-clamp. Spike numbers with the increase of injected currents in HDB cholinergic neurons recorded by whole-cell patch-clamp. f Representative traces showing sEPSC (upper) and sIPSC (lower) recorded at −60 mV (upper) and +10 mV (lower) in the same mPFC layer 2/3 pyramidal cell from Hrh1^fl/fl (black) or ChAT-Cre;Hrh1^fl/fl (red) mice. Quantification of sEPSC and sIPSC charge transfer and sEPSC/sIPSC charge transfer ratios in Hrh1^fl/fl and ChAT-Cre;Hrh1^fl/fl mice. g Representative traces showing sEPSC (upper) and sIPSC (lower) recorded at −60 mV (upper) and +10 mV (lower) in the same mPFC layer 5 pyramidal cell from Hrh1^fl/fl (black) or ChAT-Cre;Hrh1^fl/fl (red) mice. Quantification of sEPSC and sIPSC charge transfer and sEPSC/sIPSC charge transfer ratios in Hrh1^fl/fl and ChAT-Cre;Hrh1^fl/fl mice. h Threshold current to elicit action potential with the increase of injected currents in HDB cholinergic neurons treated with antipsychotic agent risperidone recorded by whole-cell patch-clamp. Spike numbers with the increase of injected currents in HDB cholinergic neurons treated with antipsychotic agent risperidone recorded by whole-cell patch-clamp. i Western blot of choline acetyltransferase (ChAT) treated with antipsychotic agent risperidone in basal forebrain. j Representative traces showing sEPSC (upper) and sIPSC (lower) recorded at −60 mV (upper) and +10 mV (lower) in the same mPFC layer 2/3 pyramidal cell from Hrh1^fl/fl + Vehicle (gray), ChAT-Cre;Hrh1^fl/fl + Vehicle (red), or ChAT-Cre;Hrh1^fl/fl treated with antipsychotic agent risperidone (purple). Quantification of sEPSC and sIPSC charge transfer and sEPSC/sIPSC charge transfer ratios in Hrh1^fl/fl + Vehicle, ChAT-Cre;Hrh1^fl/fl + Vehicle, or ChAT-Cre;Hrh1^fl/fl treated with antipsychotic agent risperidone mice. All data are presented as mean ± s.e.m. and error bars represent s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns nonsignificant. See also Supplementary Data 2 for further statistical information. Source data are provided as a Source Data file.