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. 2021 Feb 18;12:1142. doi: 10.1038/s41467-021-21476-x

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a Schematic diagram of microinjection of AAV-EF1α-DIO-hM3Dq-mCherry (hM3D-mCherry) in the HDB of BF in ChAT-Cre;Hrh1^fl/fl mice, and representative images of hM3Dq-mCherry expression in HDB ChAT⁺ cholinergic neurons. The percentage of ChAT⁺ cholinergic cells co-expressing mCherry and percentage of mCherry expressed cells co-labeling ChAT were quantified. Scale bar, 10 μm, n = 5 mice. b–g Sensorimotor gating, social behavior, hedonic function, and cognitive behavior in ChAT-Cre;Hrh1^fl/fl mice either injected with hM3Dq-mCherry or not. CNO was administrated either by i.p. injection or by intra-mPFC microinjection. b Percentage of prepulse inhibition of the auditory startle reflex across different prepulse intensities. c Time in close interaction and preference index during sociability testing (phase2), when exposed to a stranger mouse S1. Time in close interaction and preference index during subsequent social novelty recognition testing (phase 3), when exposed to a new stranger mouse S2 together with S1. d The percentage of the weight of unshredded cotton and nesting score in nest-building test. e Test for sucrose preference as a percentage of all fluid intake within a 48 h period. f Discrimination index in novel object recognition test. g Discrimination index in temporal order memory test. h Extracellular acetycholine level in the mPFC collected by microdialysis and examined by HPLC-MS/MS. All data are presented as mean ± s.e.m. and error bars represent s.e.m. *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001, ns nonsignificant. See also Supplementary Data 2 for further statistical information. Source data are provided as a Source Data file.