Table 2.
Effect of temperature, time duration, pH and hydrogen peroxide % on the decolorization of biosurfactant (ANOVA table for univariate analysis, p < 0.05).
| Tests of between-subjects effects | |||||
|---|---|---|---|---|---|
| Dependent variable: absorbance at 495 nm | |||||
| Source | Type III Sum of squares | df | Mean square | F | Sig |
| Corrected model | 3.169a | 80 | 0.040 | 6.150 | 0.000 |
| Intercept | 16.662 | 1 | 16.662 | 2.587E3 | 0.000 |
| Temperature | 0.995 | 2 | 0.497 | 77.233 | 0.000 |
| Time | 0.006 | 2 | 0.003 | 0.473 | 0.624 |
| Percentage H2O2 | 0.242 | 2 | 0.121 | 18.811 | 0.000 |
| pH | 0.153 | 2 | 0.077 | 11.913 | 0.000 |
| Temperature × time | 0.213 | 4 | 0.053 | 8.255 | 0.000 |
| Temperature × percentage H2O2 | 0.072 | 4 | 0.018 | 2.783 | 0.029 |
| Temperature × pH | 0.067 | 4 | 0.017 | 2.582 | 0.039 |
| Time × percentage H2O2 | 0.104 | 4 | 0.026 | 4.031 | 0.004 |
| Time × pH | 0.090 | 4 | 0.022 | 3.488 | 0.009 |
| Percentage H2O2 × pH | 0.076 | 4 | 0.019 | 2.965 | 0.021 |
| Temperature × time × percentage H2O2 | 0.319 | 8 | 0.040 | 6.197 | 0.000 |
| Temperature × time × pH | 0.288 | 8 | 0.036 | 5.583 | 0.000 |
| Temperature × percentage H2O2 × pH | 0.249 | 8 | 0.031 | 4.842 | 0.000 |
| Time × percentage H2O2 × pH | 0.094 | 8 | 0.012 | 1.829 | 0.075 |
| Temperature × time × Percentage H2O2 × pH | 0.200 | 16 | 0.013 | 1.944 | 0.020 |
| Error | 1.043 | 162 | 0.006 | ||
| Total | 20.874 | 243 | |||
| Corrected total | 4.212 | 242 | |||
aR Squared = 0.752 (Adjusted R Squared = 0.630)