Fig. 5.

BTF3 promotes Mfn2 expression in Wnt/β-catenin signal-dependent manner. a, b ECH enhanced TCF/LEF1 reporter gene activity, which was inhibited by CK2α′ or BTF3 knock-down. c ECH inhibited the interaction of BTF3α with TCF1/7 and LEF1. HEK293T cells were transfected with HA-tagged BTF3α and treated by ECH (10 μM) for 6 h. Co-IP was performed with anti-HA antibody, followed by immunoblotting analysis. d Cytoplasmic translocation of HA-BTF3α (green) from nucleus and nuclear translocation of β-catenin (red) from cytoplasm were promoted by ECH (scale bar: 20 μm). e IWP-2 blocked ECH-induced mitochondrial fusion; however, TWS119 did not show similar effect. Arrows (red) indicate branched healthy mitochondria. Arrows (white) indicate spherical dysfunctional mitochondria (scale bar: 20 μm). Data are expressed as the mean ± SD. ^##P < 0.01 vs. control group. *P < 0.05, **P < 0.01 vs. OGD/R group