Figure 2.
PMCA representative level of amplification for each different sCJD subtype in TgMet, TgVal and BV substrates. Serial dilutions from 10−4 to 10−9 of the different sCJD subtypes in addition to vCJD were amplified using TgMet, TgVal or BV substrates. After 4 rounds of PMCA, the PrPTSE signal was assessed by western blot analysis after proteinase K digestion using 3F4 antibody for TgMet and TgVal amplicons and 6D11 Ab for BV amplicons. For each sample, 20 µL of the product was loaded onto the gel. −3na refers to non-amplified material (no PMCA) obtained from a 10−3 dilution (w/v) of the initial infectious brain sample. N refers to substrate only amplified in the same conditions. The asterisk indicates a faint signal from incomplete PrPC digestion. M indicates the typical molecular mass of PrPres in the range of 20–30 kDa. Hatched lines correspond to cropped images.