FIGURE 2.

LH promotes FAK and paxillin phosphorylation through LHR. (A) Cells were treated with LH (5 mIU/ml) for 20 min in the presence or absence of FAKi (1 μM) and siRNA paxillin. The total cell amounts of wild-type FAK and paxillin, phospho-FAK^Y397, and p-paxillin^Y118 are shown by western blot. (B,C) Phospho-FAK^Y397 and phospho-paxillin^Y118 densitometry values were adjusted to FAK and paxillin intensity, respectively, and then normalized to the control sample. Results are expressed as the mean ± SD. *P < 0.05 vs. control. (D) BC cells were stained vs. phospho-paxillin^Y118 linked to DyLight 594 and nuclei counterstained with DAPI. (E) Quantification of the membrane-localized p-paxillin^Y118 in the different conditions. Results are expressed as dots/cells (mean ± SD). *P < 0.05 vs. CON, control. Membrane-localized p-paxillin^Y118 was counted in 40 different cells. All experiments were performed in triplicate, and representative images are shown.