FIGURE 3.

Functional characterization of Wnt pathway mutations in PDX mice. Wnt pathway activity scores were measured in tissue samples from human ovarian and breast cancer PDX (xenograft) mice. Loss of APC and gain of β-catenin (CTNNB1 gene) protein function are known to result in increased Wnt pathway activity. Wnt pathway activity scores were normal in the reference (WT), increased in loss-of-function APC-mutated ovary cancer and in gain-of-function CTNNB1 Asp32Tyr mutated breast cancer, and normal in non-functionally mutated CTNNB1 Asp665Glu breast cancer. Pathway tests were adapted for use in mouse PDX models (to exclude interference of mouse model microenvironment). Pathway Activity Scores presented on a 0–100 scale. Tissue samples were analyzed in a collaboration with Charles River Labs (CRL) (Verhaegh et al., 2018).