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Glucocorticoid receptor (NR3C1) gene methylation and expression in response to weaning. (A) Two CpG sites in the NR3C1 promoter (797 and 328 bp downstream of TSS) are significantly hypermethylated post-weaning (n = 6 animals/stage). (B) NR3C1 transcript abundance is significantly reduced post-weaning, as measured by RNA-sequencing and RT-qPCR (n = 9 animals/stage). *= < 0.05, **= < 0.01.
