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Near-infrared fluorescence imaging observation of mice with different treatment. (A) In vivo fluorescence imaging of mice after intravenous injected with free Cy5, HPMPC nanogel and RPMPC nanogel (tumor pointed out with white circle). (B) Fluorescence intensities of tumors versus post-injection time. (C) CLSM observation of tumor slices post-injection time of 24 h. The cell nuclei (blue) are stained by DAPI. Scale bar = 50 μm.
