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. 2020 Sep 19;11(2):420–433. doi: 10.1016/j.apsb.2020.09.008

Figure 3.

Figure 3

JZA02 inhibited migration and invasion of HCT-116 cells and regulated the EMT of HCT-116 cells. (A) For the scratch test, HCT-116 cells were incubated in 24-well plates with JZA01, JZA02, or IFNα and photographed at 0, 12, and 24 h. The migration lengths were measured by Image-Pro Plus 6.0 (i) and the data (ii) are presented as the mean ± SD, n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (B) Photomicrographs (i) and quantitative analysis (ii) of a Transwell invasion assay indicated that JZA02 suppressed the invasion of HCT-116 cells better than IFNα or JZA01. The count of invasive cells treated with different drugs was measured by Image-Pro Plus 6.0. Inhibition (%) = [(control–experiment)/control] × 100. (C) Western blot analysis. (i) Western blot analysis for the EMT index of HCT-116 cells that underwent treatment with PBS and JZA02. (ii) Gray scanning and data statistics of (i) based on the integrated density of β-actin. Data are presented as the mean ± SD, n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.