Figure 4.

JZA02 had superior antitumor efficacy in HCT-116-bearing nude mice and influenced the tumor microenvironment. (A) (i) Female nude mice 4–6 weeks of age (n = 5/group) were injected in the left armpit with 5 × 10⁶ HCT-116 cells and 1 × 10⁷ unstimulated hPBMCs and then were treated with JZA01 (1 mg/kg), JZA02 (1 mg/kg), IFNα (0.2 mg/kg), or JZA00+IFNα (1 mg/kg+0.2 mg/kg) twice a week, and tumor volumes were monitored for 36 days. (ii) Tumor inhibition of different groups. The data for tumor volumes are given as the mean ± SD (n = 5). (B) (i) Expression of Ki-67 in paraffin sections of xenografted tumor identified with an anti-Ki-67 antibody (brown staining). After 36 days of treatment, tumors were harvested for detected. (ii) The tumor cells that expressed Ki-67 were counted with Image-Pro-Plus. Ki-67 positive (%)=(cells that expressed Ki-67/total cells) × 100. (C) (i) The infiltration of CD8⁺ T cells was identified with an anti-CD8 antibody (brown staining). (ii) Semiquantitative and statistical analysis of the infiltration of CD8⁺ T cells. The relative number of CD8⁺ T cells was calculated with Image-Pro-Plus. All pictures were photographed by an inverted OLYMPUS microscope at 400× magnification.