Figure 5.

JZA01 had superior ability to prevent metastasis in nude mice model for liver metastasis of colorectal cancer by influencing the tumor microenvironment. (A) (i) Mice (4–6 weeks of age) were anesthetized using 3.8% (w/v) chloral hydrate (10 μL/g), and an incision was made in the middle of the abdomen. 5 × 10⁶ HCT-116-Luc-LM3 cells stably expressing firefly luciferase were injected into their spleens. After 1 week, an IVIS Imaging System was used to assess the success of engraftment and mice were divided into different treatment groups. After 21 days of treatment, an IVIS Imaging System was used to determine the extent of tumor growth (the POST-Treatment group). (ii) Tumor growth was quantified by bioluminescent imaging. (B) Livers from every group were harvested and photographed. (C) The infiltration of CD8⁺ T cells and expression of IFNγ in livers of mice was identified with an anti-CD8 antibody (red staining) and an anti-IFNγ antibody (red fluorescence). All pictures were photographed by an inverted OLYMPUS microscope at 400× magnification.