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. 2020 Sep 19;11(2):420–433. doi: 10.1016/j.apsb.2020.09.008

Figure 6.

Figure 6

Changes in protein expression associated with the effect of JZA02 treatment on metastasis. (A) IHC that was used to detect the index associated with metastasis in the liver of mice that underwent different treatments. (B) (i) Western blot that was used to detect the index associated with metastasis in the liver of mice undergoing different treatments. (ii) Gray scanning and data statistics of (i) based on the integrated density of β-actin. Data are presented as the mean ± SD, n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001. (C) (i) Western blot analysis for p-AKT/AKT and p-GSK3β/GSK3β in HCT-116-Luc-LM3 cells with treatments of PBS and JZA02. (ii) Gray scanning and data statistics of (i) based on the integrated density of β-actin. Data are presented as the mean ± SD, n = 3; ∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001.