Fig. 12.

Validation of the spatial resolution enhancement. (A) Conventional OR-PAM and PS-PAM images of a thin layer of protein molecules (BphP1), showing the finer axial resolution of RS-PAM. (B) The lateral line spread functions of OR-PAM and RS-PAM; the latter improves R_lateral from 278 nm to 141 nm. (C) The axial plane spread functions of OR-PAM and RS-PAM. The latter is capable of optical sectioning with an axial resolution of ∼410 nm; in contrast, the former’s R_axial is ∼30 μm. Figures reproduced with publisher’s permission [42].