Fig. 2. IDH2 gene deficiency accelerated unilateral ureteral obstruction (UUO)-induced mitochondrial H₂O₂ production and lipid peroxidation.

IDH2^+/+ and IDH2^−/− female mice were subjected to either UUO or a sham operation. Kidneys were harvested 5 days after the operation. (A) H₂O₂ level was measured in the kidney cell mitochondrial fraction. (B) Representative Western blots for HNE and COX IV in the mitochondrial fraction of kidneys. (C) The graph summarizes HNE expression. Data are presented as mean ± SE (n = 3). **p < 0.01 vs. its own sham-operated group; ^##p < 0.01 vs. UUO-operated IDH2^+/+ group.