Figure 10.

Confirmation of inflammation. (Left) The neutrophil activity at the site of myositis was confirmed by using i.p. administered luminol, which provides bioluminescent visualization of myeloperoxidase (MPO) enzyme activity. (Right) Histological analysis of anti-Ly6G nanoparticle uptake in mouse models. Images were captured by brightfield microscopy following staining of infected tissues using H&E staining for tissue morphology, Prussian blue staining for SPIO, and immunohistochemistry for myeloperoxidase. The Prussian blue confirmed the distribution of anti-Ly6G SPIO in bone marrow in regions with increased MPO enzyme expression (myeloid cells). The images shown in the bottom row are muscle tissues from the right infected leg. Inflammation injury was observed in the H&E stain (arrows), with the corresponding regions showing areas of increased iron and MPO enzyme activity (H&E: hematoxylin & eosin; MPO: myeloperoxidase; SPIO: superparamagnetic iron oxide (nanoparticles).