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. 2021 Feb 12;5(2):240–255. doi: 10.7150/ntno.50721

Figure 4.

Figure 4

Labeling of immune cells using anti-Ly6G SPIOs. (A) TEM images of whole blood sample incubated with anti-Ly6G SPIOs (scale bar = 2 μm). (B), (C) Magnified TEM images (scale bar = 200 nm) & (D) (scale bar = 100 nm) of yellow box marked in (A). The arrows point to the blood cell-membrane bound nanoparticles. The membrane bound particles had a core diameter of 16 ± 5 nm, corresponding well with the size of anti-Ly6G SPIO particles observed in Figure 2 (SPIO: superparamagnetic iron oxide (nanoparticles); TEM: transmission electron microscopy).