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PI3K/Akt and Ras/Raf/MAPK pathways were stably activated in Huh7R cells. (A and B) Major kinase levels of PI3K/Akt and Ras/Raf/MAPK pathways in Huh7 and Huh7R cells were examined by western blot. (C and D) Kinase expression levels in related pathways in Huh7R cells treated with sorafenib at various times were detected by western blot. Data expressed as mean ± SD of three independent experiments (*p<0.05; **p<0.01; ***p<0.001).
