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. 2021 Jan 1;17(2):417–429. doi: 10.7150/ijbs.54194

Figure 2.

Figure 2

TRIM11 depletion decreases Hippo signaling activity in anaplastic thyroid cancer cells. (A). Heatmap of top 100 differentially expressed genes. (B). Pathway enrichment of differentially expressed genes. (C). TRIM11 depletion effect by two different siRNA oligos. Anaplastic thyroid cancer cells were transfected with two independent TRIM11 siRNAs or siControl. After 48 h, TRIM11 mRNA levels were determined by qRT-PCR. 36B4 was used as internal control. (D). TRIM11 depletion decreases YAP protein level. (E, F). TRIM11 depletion decreases YAP target genes using two different siRNA oligos. (G). TRIM11 depletion affects YAP-luciferase activity. CAL62 cells were transfected with SiTRIM11 or SiControl together with YAP-luciferase reporter plasmid. Luciferase activity was measured 48 h after transfection. (H).Immunofluorescence of YAP in KHM-5M cells. *, P value < 0.05; **, P value < 0.01; ***, P value < 0.001.