Figure 3.

TRIM11 associates with YAP and increases its stability. (A) An immunofluorescence assay demonstrated that TRIM11 and YAP at least partially colocalized in CAL62 and KHM-5M cells. (B) Co-IP assay reveals association between endogenous TRIM11 and YAP in CAL62 cells. CAL62 cells were harvested with RIPA lysis buffer. Co-IP was performed using antibody as indicated. (C) In the presence of the proteasome inhibitor MG132, depletion of TRIM11 did not further decrease YAP protein levels. Anaplastic thyroid cancer cells were transfected with siTRIM11 or siControl. After 48 h, cells were treated with 10 nM MG132/vehicle for 6 h, cell lysates were prepared for western blot analysis. (D, E) TRIM11 depletion decreases YAP half-life in anaplastic thyroid cancer cells. Anaplastic thyroid cancer cells were transfected with siTRIM11 or siControl. After 48 h, cells were treated with 100 µM cycloheximide for indicated times. Cell lysates were prepared for western blot analysis.