Figure 5.

TRIM11 stabilizes YAP though its RING domain. (A) Schematic diagram of TRIM11-2CA. (B) Increasing amounts of TRIM11 WT or 2CA were transfected into CAL62 cells and YAP expression was detected. (C). TRIM11 WT or 2CA was introduced into CAL62 cells together with TRIM11 siRNA. YAP levels were measured. (B). Interaction of Myc-TRIM11-2CA proteins with endogenous YAP in CAL62 cells was analyzed by co-IP assay. (E). HEK293 cells transfected with the indicated plasmids were treated with 100 µM cycloheximide, and collected at the indicated times for western blot. (F-I) TRIM11-2CA does not modulate poly/mono-ubiquitination of YAP protein.