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. 2021 Jan 14;17(2):574–588. doi: 10.7150/ijbs.52097

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CA125 reduces DKK1 expression to activate the SGK3/FOXO3 pathway. (A) (C) (E) Western blots were performed to evaluate the expression of Akt, p-Akt, PI3K, and SGK3 in total cell lysates (TCL) and FOXO3 in cytoplasmic/nuclear protein extracts. GAPDH, Tubulin-β and PCNA were used as the experimental controls. (B) (D) (F) Relative protein expression of blots was quantified using densitometric analysis. The results represent the mean ± SD. There was no statistically significant difference.