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. 2021 Jan 25;23(4):228. doi: 10.3892/mmr.2021.11867

Figure 5.

Figure 5.

DUSP4 is a direct target gene of miR-429. (A) Binding sites between miR-429 and DUSP4 predicted by ENCORI. (B) mRNA expression levels of DUSP4 in CRC cells and resistant CRC cells, as determined by reverse transcription-quantitative PCR. ***P<0.001 vs. SW480; ###P<0.001 vs. LoVo; ∆P<0.05 vs. HCT116. (C) Protein expression levels of DUSP4 in CRC cells and resistant CRC cells were detected by western blotting. ***P<0.001 vs. SW480; ###P<0.001 vs. LoVo; ∆∆P<0.01 vs. HCT116. (D) Detection of luciferase activity. ***P<0.001 vs. mimic NC. (E) Protein expression levels of DUSP4 in resistant CRC cells post-transfection, as determined by western blotting. ***P<0.001 vs. SW480; #P<0.05 vs. SW480-R; ∆∆P<0.01 vs. SW480-R mimic NC. CRC, colorectal cancer; miR-429, microRNA-429; DUSP4, dual specificity protein phosphatase 4; WT, wild-type; MUT, mutant; NC, negative control; -R, resistant to nintedanib.