Figure 3.

YAP1 is a direct target of miR-199a. (A) Schematic of the YAP1 3′-UTR containing the miR-199a-3p binding sites. (B) miR-199a-3p inhibitor was transfected into SKOV-3 and OV90 cells and miR-199a-3p expression was determined by RT-qPCR. **P<0.01 vs. inhibitor NC in SKOV-3 cells; ^##P<0.01 vs. inhibitor NC in OV90 cells. (C) Luciferase assay of 293T cells co-transfected with firefly luciferase constructs containing the YAP1 wt or mut 3′-UTRs and miR-199a-3p mimics, mimics NC, miR-199a-3p inhibitor or inhibitor NC, as indicated (n=3). Data are presented as the mean ± standard deviation (n=3) of one representative experiment. **P<0.01 vs. mimics NC; ^##P<0.01 vs. inhibitor NC. (D) OV90 and SKOV-3 cells were transfected with miR-199a-3p mimics and mimics NC for 48 h and the protein expression levels of YAP1 were determined by western blotting. Data are presented as the mean ± standard deviation (n=3) of one representative experiment. **P<0.01 vs. mimics NC. (E) YAP1 expression was measured by RT-qPCR in 50 pairs of OC and matched adjacent non-tumor tissues. **P<0.01 vs. adjacent tissues. (F) Spearman's correlation analysis was used to analyze the correlation between YAP1 and miR-199a-3p expression in OC tissues. YAP1, Yes-associated protein 1; UTR, untranslated region; miR, microRNA; wt, wild type; mut, mutant; NC, negative control; RT-qPCR, reverse transcription-quantitative PCR.