Figure 4.

miR-150 mimic promotes PC12 cell apoptosis. (A) Transfection efficiency of miR-150 mimics and miR-150 inhibitor. (B) Cell apoptosis was determined by performing PI/Hoechst 33342 double staining. (C) Apoptotic cells were analyzed via flow cytometry. Apoptosis-related factors Apaf1, p53 and Cyto C (D) mRNA and (E) protein expression levels were determined via reverse transcription-quantitative PCR and western blotting. Experiments were repeated three times. Data are presented as the mean ± standard deviation. One-way ANOVA followed by Tukey's post hoc test was used to analyze the presented data. **P<0.01 vs. mimic NC; ^##P<0.01 vs. inhibitor NC. miR, microRNA; Apaf1, apoptotic peptidase activating factor 1; Cyto C, Cytochrome C; NC, negative control.