Figure 6.

HOXA1 overexpression suppresses miR-150-induced PC12 cell apoptosis. (A) Transfection efficiency of HOXA1 overexpression and knockdown. (B) Cell apoptosis was determined by performing PI/Hoechst 33342 double staining. (C) Apoptotic cells were analyzed via flow cytometry. Experiments were repeated three times. Data are presented as the mean ± standard deviation. One-way ANOVA followed by Tukey's post hoc test was used to analyze the presented data. **P<0.01 vs. miR-150 + empty vector; ^##P<0.01 vs. miR-150 inhibitor + scramble. HOXA1, homeobox A1; miR, microRNA; si, small interfering RNA.