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. 2021 Feb 3;23(4):254. doi: 10.3892/mmr.2021.11893

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Copyright: © Dong et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 5. — Inactivation of PI3K/AKT suppresses BMP2-induced endochondral ossification. (A) Safranin O staining at 9 days after the operation. (B) X-ray radiography for bone formation at 3 weeks after the operation. (C) Hematoxylin and eosin staining at 3 weeks after the operation. (D) Bone histomorphometric analysis, including i) the ratio of CV/TV%, ii) the BV/TV%, iii) trabecular number, iv) trabecular thickness and v) trabecular spacing (n=3). Scale bar, 100 µm. *P<0.05; **P<0.01. BMP, bone morphogenetic protein; C, cortical rim; T, trabecular structure; CV/TV, cartilage volume/tissue volume; BV/TV, ratio of bone volume/tissue volume.