Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Feb 1;23(4):245. doi: 10.3892/mmr.2021.11884

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright: © Chen et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

PMC Copyright notice

Figure 2. — SK-N-BE(2) cells deliver miR-17-5p to SH-SY5Y cells through exosomes. (A) Transmission electron micrograph of SK-N-BE(2) cell-derived exosomes. Scale bar, 100 nm. (B) Nanoparticle tracking analysis was employed for analyzing exosomes. (C) Markers of exosomes such as CD63 and TSG101 proteins, were detected by western blot assay in SK-N-BE(2) cell-derived exosomes and cell culture supernatants. (D) miR-17-5p expression in SK-N-BE(2) and SH-SY5Y cell-derived exosomes. miR-17-5p expression in SK-N-BE(2) exosomes was 7.0±1.9-fold higher compared with SH-SY5Y cells. (E) miR-17-5p expression in SH-SY5Y co-cultured with exosomes derived from SK-N-BE(2) cells via reverse transcription-quantitative PCR. **P<0.01. miR, microRNA; TSG101, tumor susceptibility gene 101 protein.