Figure 3.

Upregulation miR-17-5p enhances the proliferative and migratory capability of SH-SY5Y. (A) miR-17-5p expression was identified via reverse transcription-quantitative PCR in SH-SY5Y cells after transfection of miR-17-5p mimic or inhibitor. (B) Cell proliferation was determined using a Cell Counting Kit-8 assay. (C) Colony formation assay was employed for the determination of cell proliferation in SH-SY5Y cells. (D) Migratory ability of transfected cells was determined using a Transwell assay and the obtained results are presented as the number of migrated cells per field (magnification, ×400). (E) Detection of luciferase activity. (F) PTEN, Akt and p-Akt protein expression in SH-SY5Y cells (transfected with miR-17-5p mimics or inhibitor) were measured using western blotting. The results are presented as the average of three experiments run individually (mean ± SD). *P<0.05, **P<0.01 and ***P<0.001 vs. NC mimic or NC inhibitor. NC, negative control; miR, microRNA; UTR, untranslated region; mut, mutant; wt, wild-type; p-, phosphorylated.