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Differential gene expression in key signaling pathways shaping MN differentiation. Gene transcript levels were determined by global RNA-seq analysis of (a) TGFβ, (b) Notch, and (c) Sonic Hedgehog (Shh) pathways and (d) Calcium signaling. Fold change was calculated relative to RNA reads in iPSC (D0). The statistics (average value and standard error) were derived from three independent biological replicas with p < 0.001 determined by R-statistical analysis package
