Table 5.
DEGs identified in KEGG pathway: Cholinergic synapse. Differential expression indicates the average log2-fold change in RNA-Seq transcript levels from three independent experiments at each sampling period: D7 vs D0, D13 vs D7, D18 v D13, and D28 v D18. The p-values were ≤ 0.001. Negative log2-fold change corresponds to gene downregulation and the positive values indicate gene activation
| Cholinergic synapse | |||
|---|---|---|---|
| Gene ID | Description | Differential expression | Time |
| GNG12 | guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-12 | −2.18897 | D0-D7 |
| CACNA1B | voltage-dependent calcium channel N type alpha-1B | −1.8346 | D0-D7 |
| KCNQ3 | potassium voltage-gated channel KQT-like subfamily member 3 | −2.96129 | D0-D7 |
| KCNJ12 | potassium inwardly-rectifying channel subfamily J member 12 | 3.350697 | D0-D7 |
| CHRNA3 | nicotinic acetylcholine receptor alpha-3 | 2.793838 | D0-D7 |
| CHRNB4 | nicotinic acetylcholine receptor beta-4 | 4.925251 | D0-D7 |
| ITPR2 | inositol 1,4,5-triphosphate receptor type 2 | −3.69982 | D0-D7 |
| ITPR3 | inositol 1,4,5-triphosphate receptor type 3 | −2.4065 | D0-D7 |
| ADCY2 | adenylate cyclase 2 | −3.4602539 | D0-D7 |
| ADCY6 | adenylate cyclase 6 | 1.892735 | D0-D7 |
| CREB5 | cyclic AMP-responsive element-binding protein 5 | 2.033579 | D0-D7 |
| FYN | tyrosine-protein kinase Fyn | 1.772436 | D0-D7 |
| CAMK4 | calcium/calmodulin-dependent protein kinase IV | 1.419104 | D7-D13 |
| ChAT | choline O-acetyltransferase | 4.392642 | D13-D18 |
| CACNA1B | voltage-dependent calcium channel N type alpha-1B | 1.880481 | D13-D18 |
| SLC5A7 | solute carrier family 5 (high affinity choline transporter), member 7 | 4.94003258 | D13-D18 |
| KCNQ2 | potassium voltage-gated channel KQT-like subfamily member 2 | 3.013059 | D13-D18 |
| CHRNA4 | nicotinic acetylcholine receptor alpha-4 | 2.830329 | D13-D18 |
| CHRM3 | muscarinic acetylcholine receptor M3 | 3.97881916 | D18-D28 |
| ChAT | choline O-acetyltransferase | 1.799457 | D18-D28 |
| ACHE | Acetylcholinesterase | 2.982588 | D18-D28 |
| CHRM2 | muscarinic acetylcholine receptor M2 | 3.383752 | D18-D28 |
| SLC5A7 | solute carrier family 5 (high affinity choline transporter), member 7 | 2.363264 | D18-D28 |
| KCNQ3 | potassium voltage-gated channel KQT-like subfamily member 3 | 3.701006 | D18-D28 |
| CHRNA3 | nicotinic acetylcholine receptor alpha-3 | −2.67044 | D18-D28 |
| PRKCB | classical protein kinase C beta type | 1.642454 | D18-D28 |
| ADCY2 | adenylate cyclase 2 | 3.240853 | D18-D28 |