Table 6.
MN specification based on log2-fold change in RNA-Seq transcript levels at D28 versus D0 of the differentiation protocol. The p-values were calculated with the R-package. Negative log2-fold change corresponds to gene downregulation and the positive values indicate gene activation. ↓ indicates the gene is expected to be downregulated in the MN subtype
| Gene Biomarkers | Fold change | p-value | |
|---|---|---|---|
| General motor neuron | HB9 | 4.29740102 | 0.03478379 |
| ISL1 | 9.38120075 | 1.39E-11 | |
| ISL2 | −2.71940491 | 0.12115956 | |
| LHX3 | 5.98518477 | 1.09E-05 | |
| Hypaxial motor column | ETV1 | −4.14432026 | 8.33E-05 |
| HB9 | 4.29740102 | 0.03478379 | |
| ISL1 | 9.38120075 | 1.39E-11 | |
| ISL2 | −2.71940491 | 0.12115956 | |
| Pre-ganglionic column | SMAD1 | 1.61250783 | 3.71E-10 |
| NOS1 | −1.1688341 | 0.1409026 | |
| ZEB2 | 4.94457944 | 9.71E-19 | |
| FOXP1 | 0.76366376 | 0.10008361 | |
| Spinal accessory column | ALCAM | 0.82274029 | 0.14709124 |
| ISL1 | 9.38120075 | 1.39E-11 | |
| RUNX1 | 4.90888056 | 1.01E-20 | |
| PHOX2B | 9.18779756 | 1.30E-06 | |
| Medial LMC | ↓ HB9 | 4.29740102 | 0.03478379 |
| ISL2 | −2.71940491 | 0.12115956 | |
| FOXP2 | 5.83531355 | 9.82E-56 | |
| ALDH1A2 | 5.96267067 | 8.55E-13 | |
| Lateral LMC | ↓ ISL1 | 9.38120075 | 1.39E-11 |
| ISL2 | −2.71940491 | 0.12115956 | |
| FOXP2 | 5.83531355 | 9.82E-56 | |
| ALDH1A2 | 5.96267067 | 8.55E-13 | |
| Median motor column | HB9 | 4.29740102 | 0.03478379 |
| ISL1 | 9.38120075 | 1.39E-11 | |
| ISL2 | −2.71940491 | 0.12115956 | |
| LHX3 | 5.98518477 | 1.09E-05 |