Table 2.

Laboratory findings of selected human samples underwent IFA confirmation

Sample collection sites	Total samples	Positive by RDT (%)	Positive by IgM ELISA (%)	Positive by IFA (%)
NPHL	50	NT	30 (60.0)	26 (52.0)
Bharatpur hospital	11	3 (27.3)	NT	3 (27.3)

A fraction of samples (n = 61) from suspected scrub typhus cases were randomly selected and sent to reference laboratory (Armed Forces Research Institute of Medical Sciences, Bangkok, Thailand) for IFA confirmation. RDT, rapid diagnostic test; ELISA Enzyme linked immune-sorbent assay; IFA, Indirect fluorescence antibody assay; NPHL National Public Health Laboratory; NT Not tested