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. 2021 Feb 19;22:66. doi: 10.1186/s13059-021-02288-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — flsnRNA-seq captures the variation in intron retention levels of different clusters. a UMAP visualization of clustering result using Illumina single-nucleus data (left panel), and cartoon illustration of major cell types in Arabidopsis endosperm at heart stage (right panel). b UMAP visualization of incompletely spliced ratio calculated by Nanopore full-length reads. c Barplot visualization of the incompletely spliced ratio of each cluster. Differences in incompletely spliced ratios between each cluster to all other clusters were tested using a one-sided Kolmogorov-Smirnov test. “***” denotes that the p value is lower than 0.001. d Quantification of nuclei with each cell type per cluster. The number represents nucleus counts and the color represents the proportion of cell types in each cluster. e GO term enrichment analysis of all 93 enriched genes for cluster 4. Only cellular component terms are plotted. “*” and “***” denote that the adjusted p value is lower than 0.05 and 0.001, respectively