FIGURE 2.

Junctional adhesion molecule‐A (JAM‐A) contributes to proliferation, colony formation and collective migration of uterine cervical adenocarcinoma cells. A, JAM‐A was expressed in uterine cervical adenocarcinoma cell lines. B, KO of JAM‐A expression was achieved by using the CRISPR/cas9 system in HCA1 cells. C, WST‐8 assay (5000 cells/well). Proliferation of HCA1 cells was significantly inhibited by KO of JAM‐A. D, Colony formation assay (6‐well plate, 1250 cells/well). Number of colonies of HCA1 cells was decreased by KO of JAM‐A. E, F, Immunohistochemistry of Ki‐67 (proliferation marker) and cleaved caspase‐3 (apoptosis marker) in cell block samples of HCA1 cells. KO of JAM‐A significantly decreased the number of Ki‐67‐positive cells and increased the number of cleaved caspase‐3‐positive cells (Ki‐67: n = 5, cleaved caspase‐3: n = 6). The number of positive cells per 100 cells is represented as mean ± SD. G, Wound healing assay. Wound closure was significantly inhibited by KO of JAM‐A. **P < .01 vs control cells. A, B, western blot analysis