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. 2020 Dec 22;112(2):589–603. doi: 10.1111/cas.14764

FIGURE 7.

FIGURE 7

Effects of miR‐182 overexpression on intracellular expression of FoxP3 and IL‐17. A, Graph of the cell population by using FSC‐A versus SSC‐A plot in unstained control group stimulated with PMA/ionomycin and brefeldin A. B, FOXP3+ population in unstained control group stimulated with PMA/ionomycin and brefeldin A that was stained for intracellular FOXP3. C, FOXP3+ population in mock control group stimulated with PMA/ionomycin and brefeldin A that was stained for intracellular FOXP3. D, Quadrant showing the population of FOXP3‐IL17+ T cells (Q1), FOXP3+IL17+ T cells (Q2), FOXP3+IL17‐ T cells (Q3), and FOXP3‐IL17‐ T cells (Q4) in the control group (mock) stimulated with PMA/ionomycin and brefeldin A that was stained for intracellular FOXP3 and IL‐17. E, Population of cells transduced by miR‐182 that expressed intracellular FOXP3. F, Population of cells transduced by miR‐182 and stimulated with PMA/ionomycin and brefeldin A that was stained for intracellular FOXP3 and IL‐17. G, Percentage of FOXP3+ T cells after transduction by miR‐182 and stimulation with PMA/ionomycin and brefeldin A that was stained for intracellular FOXP3 and IL‐17. H, Percentage of FOXP3+ IL17‐producing T cells after transduction by miR‐182 and stimulation with PMA/ionomycin and brefeldin A that were stained for intracellular FOXP3 and IL‐17 (***P < .001). FSC‐A, forward scatter‐A; SSC‐A, side scattering; FITC‐A::FOXP3, Anti‐FOXP3‐FITC‐A; APC‐A::IL‐17, Anti‐IL‐17‐APC‐A