| Primers for qRT‐PCR |
| miR ‐182‐5p |
5′‐GTGCAGGGTCCGAGGT‐3′ |
5′‐GATTTGGCAATGGTAGAACTCAC‐3′ |
| miR ‐182‐3p |
5′‐GTGCAGGGTCCGAGGT‐3′ |
5′‐CAATGGTTCTAGACTTGCCAACT‐3′ |
| miR‐183‐5p |
5′‐GTGCAGGGTCCGAGGT‐3′ |
5′‐ATACTATGGCACTGGTAGAATTCACT‐3′ |
| miR‐96‐5p |
5′‐GTGCAGGGTCCGAGGT‐3′ |
5′‐TAATTTGGCACTAGCACATTTTTGC‐3′ |
| RNU6 |
5′‐CGCTTCACGAATTTGCGTGTC‐3′ |
5′‐CGCTTCGGCAGCACATATACT‐3′ |
| Gapdh |
5′‐CCGAGCCACATCGCACAG‐3′ |
5′‐GGCAACAATATCCACTTTACCAG‐3′ |
| Β‐actin |
5′‐AGACGCAGGATGGCATGGG‐3′ |
5′‐GAGACCTTCAACACCCCAGCC‐3′ |
| CD3D |
5′‐AAGTGAGCCCCTTCAAGATACC‐3′ |
5′‐TCTGAGAGCAGTGTTCCCAC‐3′ |
| ITK |
F5′‐ACTCCTGAAGACAACAGGCGA‐3′ |
5′ATCCTTCATGCCCATTCCTGTC‐3′ |
| NFATC3 |
5′‐GTGAAGCTCCTGGGCTATAACG‐3′ |
5′‐TATCTCTTGGCTTGCAGTAGCG‐3′ |
| NFATC4 |
5′‐GAAGGGTGAGACGGACATCG‐3′ |
5′‐TTGGAGCCAGTCAGTACCAGT‐3′ |
| IL‐2 |
5′‐AAGGCCACAGAACTGAAACATC‐3′ |
5′‐ATTGCTGATTAAGTCCCTGGGT‐3′ |
| TNF‐α |
5′‐ATGTTGTAGCAAACCCTCAAGC‐3′ |
5′‐AGGACCTGGGAGTAGATGAGG‐3′ |
| TGF‐β |
F5′‐ AGTGGACATCAACGGGTTCAC‐3′ |
5′‐TGGAGCTGAAGCAATAGTTGGTG‐3′ |
| IL‐6 |
5′‐ GCACTGGCAGAAAACAACCT‐3′ |
5′‐ CAGGGGTGGTTATTGCATCT‐3′ |
| IL‐2RA |
5′‐ GATGCCAAAAAGAGGCTGACG‐3′ |
5′‐CCACATCAGCAGGTATGAATCCA‐3′ |
| FOXO1 |
5′‐ GAGGGTTAGTGAGCAGGTTACAC‐3′ |
5′‐ TGCTGCCAAGTCTGACGAAAG‐3′ |
| FOXP3 |
5′‐TTCATCTGTGGCATCATCCGAC‐3′ |
5′‐ GTCGCATGTTGTGGAACTTGAAG‐3′ |
| Primers for cloning |
| MIR‐182 |
5′‐TTGGAAGGAGTTCGACCCTGCAGGAAGGACCTTG‐3′ |
5′‐GTTGCGGCCGCGGATGCAGGGAAACACAGAGTG‐3′ |
