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. 2020 Dec 28;112(2):703–714. doi: 10.1111/cas.14743

FIGURE 2.

FIGURE 2

Suppression of epithelial cell transforming sequence 2 (ECT2) inhibits the spreading of lung adenocarcinoma (LAC) cells. Calu‐3 and NCI‐H2342 cells transfected with siECT2#1 or siCON were spread on COLl‐coated plates for 12 and 24 h. A, Phase‐contrast micrographs show that the cells treated with siECT2#1 exhibit delayed or absent spreading relative to control cells. B, The area of spread of transfected cells was analyzed and quantified using ImageJ software. Error bars represent mean ± SD, n = 4, scale bar 100 μm, **P < .01, ***P < .001