FIGURE 6.

Knockdown of focal adhesion kinase (FAK) regulates the proliferation, adhesion, and spreading of lung adenocarcinoma (LAC) cells. Calu‐3 and NCI‐H2342 were transfected with siFAK#1 and siFAK#2. Knockdown efficiency was confirmed at both the mRNA and protein levels A, B. Error bars represent mean ± SD, n = 3. C, Cell proliferation of the Calu‐3 and NCI‐H2342 cells. Error bars represent mean ± SD from more than nine independent experiments. D, Calu‐3 and NCI‐H2342 cells were transfected with siFAK#1 or siCON and then plated on COLl‐ or 1% BSA‐coated plates for 90 min. Error bars represent mean ± SD, n = 3. E, The area of spread of transfected Calu‐3 and NCI‐H2342 cells was analyzed and quantified using ImageJ software. Error bars represent mean ± SD, n = 4, *P < .05, **P < .01, ***P < .001