FIGURE 3.

The impact of histone‐PTMs on the TSPY1 promoter activity. A‐C, ChIP‐qPCR analysis comparing the enrichment level of the TSPY1 promoter region occupied by the histone‐PTM in different tissues and cells, including testis and blood (A), TSPY1 expression and non‐expression PCa (B), and LNCaP and DU145 (C). D and E, Expression of TSPY1 mRNA was activated in 22RV1 (D) and DU145 (E) cells that were treated with the HDAC inhibitor trichostatin A (TSA). F, TSA treatment alone activated the TSPY1 expression in 22RV1 cells. TSA further increased the TSPY1 expression in 22RV1 cells to a higher level than treatment with AZA alone. G, The DNA methylation level of the TSPY1 CGI in 22RV1 cells that were treated with TSA, AZA alone, or a combination of AZA and TSA