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. 2020 Dec 15;112(2):679–690. doi: 10.1111/cas.14729

Figure 5.

Figure 5

Histone H3 K9 acetylation is positively correlated with high‐mobility group protein A2 (HMGA2) expression in hepatocellular carcinoma (HCC). A, Western blot analysis shows that acetyl‐H3 K9 expression was upregulated in the livers of diethylnitrosamine (DEN)‐treated rats. **P < .01 compared to normal rats, two‐tailed unpaired t test, n = 8 per group. B, Immunohistochemistry (IHC) suggested that acetyl‐H3 expression in the livers of DEN‐rats was enhanced compared to normal rats, **P < .01, two‐tailed unpaired t test, n = 8 per group. Scale bar, 100 µm. C, Representative photos of IHC of DEN‐treated rat livers with anti‐acetyl‐H3‐K9 and HMGA2 antibodies. Scale bar, 100 µm. D, Level of H3‐K9 acetylation correlated with the expression levels of HMGA2 in DEN‐treated rats; Pearson product moment correlation test. E, Representative photos of IHC of 39 HCC specimens with anti‐acetyl‐H3‐K9 and HMGA2 antibodies. Scale bar, 100 µm. F, Level of H3‐K9 acetylation correlated with the expression levels of HMGA2. Pearson product moment correlation test; note that some of the dots on the graphs represent more than one specimen (ie, some scores overlapped)