Figure 1.

Steroid receptor coactivator‐1 (SRC‐1) expression levels positively correlated with clinical glioma grades and inversely correlated with glioma patient prognosis. A, Western blot analysis for SRC‐1 in U87MG, U251, U118, T98G, LN229, C6, and SVG p12 cells. B, Clinical glioblastoma (GBM) tissue stained for glial fibrillary acidic protein (GFAP) (red) and SRC‐1 (green) by IF analysis. Scale bar, 50 μm. C, Relationship between clinicopathologic variables and SRC‐1 expression. D, SRC‐1 protein levels of low grade (Ⅰ, Ⅱ) and high grade (Ⅲ, Ⅳ) of GBM were compared using the immunoreactive score. P values were calculated using Fisher’s exact test. ***P < .001. E, Representative images of immunohistochemical staining of SRC‐1 in glioma tissues. Scale bar, 100 μm. F, G, Overall survival of 85 glioma patients in the GSE4412 cohort and 77 glioma patients in the GSE4271 cohort