Figure 2.

Steroid receptor coactivator‐1 (SRC‐1) promotes the cell proliferation of glioblastoma cells. A‐D, Cell viability was examined by MTT assay for U251, U87, C6, and LN229 cells in 48 h (n = 3). E, F, Cell colony formation ability was examined in U251 and LN229 cells (n = 3). G, I, Flow cytometry analysis was used to examine cell cycles in U251 and LN229 cells (n = 3). PI, propidium iodide. H, J, Western blot analysis for SRC‐1, proliferating cell nuclear antigen (PCNA), cyclin D1, and cyclin‐dependent kinase 4 (CDK4) in U251, U87, C6, and LN229 cells. K, L, Cell viability was detected in U251 cells, which were treated with temozolomide (TMZ; 500 and 1000 μmol/L) and sorafenib (SOR; 50 and 80 μmol/L). *P < .05, **P < .01