Figure 4.

Steroid receptor coactivator‐1 (SRC‐1) enhances the self‐renewal ability of glioblastoma stem cells. A‐D, Self‐renewal ability was detected in U251, U87, C6, and LN229 cells by sphere formation assay. Scale bar, 100 μm. E, Representative images and quantitative assessment of CD133 were examined in LN229‐SRC1 spheres compared with LN229‐EV spheres using flow cytometry. F, G, Expression of SRC‐1, CD133, and Kruppel‐like factor 4 (KLF4) were detected by western blot analysis in U251, U87, C6, and LN229 cells. *P < .05, **P < .01, ***P < .001