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. 2020 Dec 14;112(2):604–618. doi: 10.1111/cas.14685

Figure 5.

Figure 5

Overexpression of steroid receptor coactivator‐1 (SRC‐1) increases glioblastoma (GBM) tumorigenesis and SRC‐1 knockdown inhibits orthotopic GBM growth in vivo. A, Tumor volume was measured and calculated. n = 6. B, Tumor weight was measured in 8 weeks. n = 6. C, Western blot analysis for SRC‐1, Kruppel‐like factor 4 (KLF4), Vimentin, and proliferating cell nuclear antigen (PCNA) in LN229‐EV and LN229‐SRC1 tumors. D, H&E staining showing the tissue morphology and immunohistochemical (IHC) staining showing the SRC‐1 and PCNA expression pattern in xenograft tumors of nude mice. Scale bar, 100 μm. E, Pathological picture of orthotopic gliomas after 28 days. F, Glioma volume was measured and calculated after 28 days. n = 6. G, Representative H&E staining showing the tissue morphology. Scale bar, 1 mm and 20 μm. H, IHC staining showing the SRC‐1, PCNA, and CD133 expression patterns in rat brain orthotopic glioma. I, Western blot analysis for SRC‐1, KLF4, Vimentin, PCNA, and CD133 in normal brain and glioma tissues. *P < .05, **P < .01